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Rhodamine x

JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser. T has been modified to contain covalently linked X-rhodamine at random surface lysines. An activated ester of X-rhodamine was used to label the protein. Labeling stoichiometry was determined by spectroscopic measurement of protein and dye concentrations dye extinction coefficient when protein bound is 66,M -1 cm Final labeling stoichiometry is dyes per tubulin heterodimer. X-rhodamine labeled tubulin can be detected using a filter set of nm excitation and emission. X-rhodamine tubulin is in a versatile, stable and easily shipped format. It is ready for micro-injection or in vitro polymerization. Cytoskeleton, Inc.
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This is not about either of you individually. How the Book of Mormon was translated using a sacred rock. Things are very very difficult at the moment. I get that a lot to people saying 'be happy you married a doctor' 'u will have a glamorous wonderful life' they would not get it unless they be one-that is being a docs wife, the loneliness is the worst especially when your newly wedded and 2nd month of ur marriage he has to go for his night calls in dec, which includes our first christmas: I thought I was the only one who barely gets communication throughout the day, not married but am dating a surgeon and have for 6 years. She still has another year of residency but I am hoping she chooses a specialty that is not as demanding on her time. I can only say that I was that girl, 10 years ago. Next year we are getting married but I already see a tough life ahead of me. He is absolutely, hands-down my favorite human being on the planet. I trust him and I highly doubt he's getting "it" from someone else. Keep things going and see what happens.

And I resent being viewed as just the wife of a doctor rather than my own person with her own accomplishments and aspirations. These are things your wife may consider matter-of-fact and you may be surprised by if you don't discuss them now. I made a conscious decision to marry outside the church for my own reasons. So good to find this I am married to a Dr with 2 young kids. Tips Mormon girls are much like other girls their age, in that they want guys to respect them and treat them well. He is in a way to become a Ex mormon. I can no longer spend more than 30 minutes in the sun. You know what the official line of the church is, and what bishops and stake presidents are likely to say. From her summary, he just did not understand her dilemma at all. So do you just never give anything back to SOs in terms of time or small gestures.

JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser. T has been modified to contain covalently linked X-rhodamine at random surface lysines.

An activated ester of X-rhodamine was used to label the protein. Labeling stoichiometry was determined by spectroscopic measurement of protein and dye concentrations dye extinction coefficient when protein bound is 66,M -1 cm Final labeling stoichiometry is dyes per tubulin heterodimer. X-rhodamine labeled tubulin can be detected using a filter set of nm excitation and emission. X-rhodamine tubulin is in a versatile, stable and easily shipped format.

It is ready for micro-injection or in vitro polymerization. Cytoskeleton, Inc. TLM , rhodamine Cat. TLM labeled tubulins of the same quality. Labeled protein is run on an SDS gel and photographed under green light. No fluorescence is detected in the dye front, indicating that no free dye is present in the final product Figure 1 B. Biological Activity The biological activity of X-rhodamine tubulin is assessed by a tubulin polymerization assay.

This is comparable to unlabeled tubulin under identical conditions. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice cytoskeleton.

Ho et al. Interaction of antiparallel microtubules in the phragmoplast is mediated by the microtubule-associated protein MAP in Arabidopsis. Plant Cell. TLM be used to monitor tubulin dynamics in living cells? Please see the brief protocol on the product datasheet Cat.

TLM and these papers for guidance on micro-injecting cells with fluorescently-labeled proteins Smilenov et al. Focal adhesion motility revealed in stationary fibroblasts. Protein kinase C-d C2-like domain is a binding site for actin and enables actin redistribution in neutrophils. Live cell imaging of F-actin dynamics via fluorescent speckle microscopy FSM. Under these conditions the protein is stable for 6 months.

NOTE: It is very important to snap freeze the tubulin in liquid nitrogen as other methods of freezing will result in significantly reduced activity. Defrost rapidly by placing in a room temperature water bath for 1 min. If you have any questions concerning this product, please contact our Technical Service department at tservice cytoskeleton. Tubulin protein X-rhodamine : porcine brain. Tubulin protein X-rhodamine : bovine brain.

Amount 5x20 ug Cat. Submit Order Request Contact Distributor. Add to Wish List. X-rhodamine microtubules formed from X-rhodamine labeled tubulin. Related Products. Tubulin protein biotin : porcine brain. Tubulin protein rhodamine : porcine brain. Tubulin protein fluorescent HiLyte : porcine brain.

GTP: mM stock. Paclitaxel Taxol. General tubulin buffer: 1x stock. Tubulin glycerol buffer: cushion buffer. Actin protein biotin : skeletal muscle. ATP: mM stock.



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