An activated ester of X-rhodamine was used to label the protein. Labeling stoichiometry was determined by spectroscopic measurement of protein and dye concentrations dye extinction coefficient when protein bound is 66,M -1 cm Final labeling stoichiometry is dyes per tubulin heterodimer. X-rhodamine labeled tubulin can be detected using a filter set of nm excitation and emission. X-rhodamine tubulin is in a versatile, stable and easily shipped format.
It is ready for micro-injection or in vitro polymerization. Cytoskeleton, Inc. TLM , rhodamine Cat. TLM labeled tubulins of the same quality. Labeled protein is run on an SDS gel and photographed under green light. No fluorescence is detected in the dye front, indicating that no free dye is present in the final product Figure 1 B. Biological Activity The biological activity of X-rhodamine tubulin is assessed by a tubulin polymerization assay.
This is comparable to unlabeled tubulin under identical conditions. For additional information, click on the FAQs tab above or contact our Technical Support department at tservice cytoskeleton.
Ho et al. Interaction of antiparallel microtubules in the phragmoplast is mediated by the microtubule-associated protein MAP in Arabidopsis. Plant Cell. TLM be used to monitor tubulin dynamics in living cells? Please see the brief protocol on the product datasheet Cat.
TLM and these papers for guidance on micro-injecting cells with fluorescently-labeled proteins Smilenov et al. Focal adhesion motility revealed in stationary fibroblasts. Protein kinase C-d C2-like domain is a binding site for actin and enables actin redistribution in neutrophils. Live cell imaging of F-actin dynamics via fluorescent speckle microscopy FSM. Under these conditions the protein is stable for 6 months.
NOTE: It is very important to snap freeze the tubulin in liquid nitrogen as other methods of freezing will result in significantly reduced activity. Defrost rapidly by placing in a room temperature water bath for 1 min. If you have any questions concerning this product, please contact our Technical Service department at tservice cytoskeleton. Tubulin protein X-rhodamine : porcine brain. Tubulin protein X-rhodamine : bovine brain.
Amount 5x20 ug Cat. Submit Order Request Contact Distributor. Add to Wish List. X-rhodamine microtubules formed from X-rhodamine labeled tubulin. Related Products. Tubulin protein biotin : porcine brain. Tubulin protein rhodamine : porcine brain. Tubulin protein fluorescent HiLyte : porcine brain.
GTP: mM stock. Paclitaxel Taxol. General tubulin buffer: 1x stock. Tubulin glycerol buffer: cushion buffer. Actin protein biotin : skeletal muscle. ATP: mM stock.